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In a second experiment, primary Hupki cells were exposed to the carcinogen aristolochic acid I (AAI).
The Affymetrix p53 Gene Chip assay also readily identified the 6 single-base substitutions.
All mutations in HUFs from UV-treated cultures were at dipyrimidine sites, including 3 nontranscribed strand C →T transitions.
The mutant HUFs were deficient in p53 transactivation function, and missense mutants had high levels of nuclear p53 protein.
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To date, there has been no way to examine induced human p53 gene mutations in cell cultures exposed to mutagenic factors, other than by restriction site analysis.